GeneTex
United States (US)

CD21 antibody [1F8]

CD21-antibody-1F8-GTX29492-IHC-1_18121409_435.jpg
CD21-antibody-1F8_IHC (Formalin-fixed paraffin-embedded sections)_GTX29492-1_18121409_448.jpg

Cat No. GTX29492

Host Mouse
Clonality Monoclonal
Clone Name 1F8
Isotype IgG1
Application IHC-P, IHC-Fr, IHC
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60 mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user.

Calculated MW

113 kDa. ( Note )

Positive Control

Tonsil

Specificity/Sensitivity

This antibody reacts with CD21 antigen of MW 145 kDa, present on the surface of mature B cells. The antigen is present at high density on follicular dendritic cells. CD21 is involved in transmitting growth promoting signals to the interior of the B cell.
PROPERTIES

Form

Liquid

Buffer

This antibody contains sodium azide as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified receptor for the C3d fragment of the third complement component CR2.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD21, 120650, 1380, CR2, P20023, C3DR, SLEB9

Cellular Localization

Cell membrane.

Background

CD21 also known as complement receptor 2 (CR2). C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23-signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.

Database

Research Area

DATA IMAGES
CD21-antibody-1F8-GTX29492-IHC-1_18121409_435.jpg

GTX29492 IHC Image

IHC image using GTX29492 - Detection of CD21 by IHC in human tonsil

CD21-antibody-1F8_IHC (Formalin-fixed paraffin-embedded sections)_GTX29492-1_18121409_448.jpg

GTX29492 IHC-P Image

Formalin fixed paraffin embedded human tonsil stained with CD21 antibody (cat. # GTX73597).

Package List Price ($)
$ 289