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CD22 antibody [BLCAM/1795]

Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478

Cat No. GTX34478

Host Mouse
Clonality Monoclonal
Clone Name BLCAM/1795
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd22 Molecule,Siglec-2,Siglec2,Cd22

Database

Research Area

DATA IMAGES
Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478

GTX34478 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).

Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478

GTX34478 WB Image

Western Blot Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).

Package List Price ($)
$ 329