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CD22 antibody [BLCAM/1795]

Anti-CD22 antibody [BLCAM/1795] used in Protein Array (Protein Array). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in Flow cytometry (FACS). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478
Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478

Cat No. GTX34478

Host

Mouse

Clonality

Monoclonal

Clone Name

BLCAM/1795

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD22 , CD22 molecule , SIGLEC2

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-CD22 antibody [BLCAM/1795] used in Protein Array (Protein Array). GTX34478

GTX34478 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478

GTX34478 IHC-P Image

IHC-P analysis of human spleen tissue using GTX34478 CD22 antibody [BLCAM/1795].

Anti-CD22 antibody [BLCAM/1795] used in IHC (Paraffin sections) (IHC-P). GTX34478

GTX34478 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34478 CD22 antibody [BLCAM/1795].

Anti-CD22 antibody [BLCAM/1795] used in Flow cytometry (FACS). GTX34478

GTX34478 FACS Image

FACS analysis of paraformaldehyde-fixed MOLT4 cells using GTX34478 CD22 antibody [BLCAM/1795].
Blue : Primary antibody
Red : Isotype control

Anti-CD22 antibody [BLCAM/1795] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34478

GTX34478 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed Ramos cells using GTX34478 CD22 antibody [BLCAM/1795].
Green : Primary antibody
Red : nucleus

Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478

GTX34478 WB Image

WB analysis of Raji and Ramos cell lysates using GTX34478 CD22 antibody [BLCAM/1795].

Anti-CD22 antibody [BLCAM/1795] used in Western Blot (WB). GTX34478

GTX34478 WB Image

WB analysis of Raji cell lysate using GTX34478 CD22 antibody [BLCAM/1795].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339