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CD226 antibody [DX11] (Low endotoxin, azide free)

Cat. No. GTX42241

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DX11

Isotype

 IgG1

Application

 FACS, IP, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all CD226 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1/50-1/200
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003).
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human cytotoxic T lymphocyte clone.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD226 molecule , DNAM-1 , DNAM1 , PTA1 , TLiSA1

Cellular Localization

Cell membrane

Background

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Database

Research Area

REFERENCE

Clone Reference

Banta KL et al. Immunity 2022; 55 (3):512-526.e9 Mechanistic convergence of the TIGIT and PD-1 inhibitory pathways necessitates co-blockade to optimize anti-tumor CD8+ T?cell responses.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations