PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Positive Control
Tonsil: The follicular dendritic cells of the germinal centres should show a strong CD23 immunostaining while the majority of lymphocytes in the mantle zone of the follicles should show an at least weak staining.
Negative Control
Tonsil: The squamous epithelial cells and T-cells in the interfollicular T-zones should not show any CD23 immunostaining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fc fragment of IgE receptor II , BLAST-2 , CD23 , CD23A , CLEC4J , FCE2 , IGEBF
Cellular Localization
Cell membrane; Single-pass type II membrane protein , Secreted
Background
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Database
Research Area
DATA IMAGES
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/templates/default/js/lightbox/images/loading.gif "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
|
GTX04472 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX04472 CD23 antibody [MSVA-023M] HistoMAX™.
In lymph nodes a strong CD23 immunostaining is seen in follicular dendritic cells of germinal centres while staining is moderate in follicular and perifollicular B lymphocytes.
|
|
|
GTX04472 IHC-P Image
IHC-P analysis of human B-cell chronic lymphocytic leukemia (B-CLL) tissue using GTX04472 CD23 antibody [MSVA-023M] HistoMAX™.
B cell chronic lymphatic leukemia (B-CLL) composed of strongly CD23 positive tumor cells.
|
|
|
GTX04472 IHC-P Image
IHC-P analysis of human mantle cell lymphoma (MCL) tissue using GTX04472 CD23 antibody [MSVA-023M] HistoMAX™.
CD23 negative mantle cell lymphoma containing few germinal centre remnants that include CD23 positive dendritic cells.
|
REFERENCE
There are currently no references for CD23 antibody [MSVA-023M] HistoMAX™ (GTX04472). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD23 antibody [MSVA-023M] HistoMAX™ (GTX04472). Be the first to share your experience with this product.
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/small/GTX04472/GTX04472_20230728_IHC-P_18_23072722_301.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/small/GTX04472/GTX04472_20230728_IHC-P_148_23072722_409.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/small/GTX04472/GTX04472_20230728_IHC-P_278_23072723_647.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/normal/GTX04472/GTX04472_20230728_IHC-P_18_23072722_301.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/normal/GTX04472/GTX04472_20230728_IHC-P_148_23072722_409.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")
![Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472](/upload/website/prouct_img/normal/GTX04472/GTX04472_20230728_IHC-P_278_23072723_647.webp "Anti-CD23 antibody [MSVA-023M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04472")