APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:1000 |
| 1:50-1:200 |
| 1:50-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CD27 molecule , CD27 , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55
Cellular Localization
Membrane
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_HM1119_20200317_WB_20032502_471.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
|
GTX01122 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD27 antibody [JB40-98] (GTX01122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_HM1119_20200814_WB_R_spleen_20082000_593.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
|
GTX01122 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD27 antibody [JB40-98] (GTX01122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_20200303_IHC-P_494_20030422_713.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
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GTX01122 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX01122 CD27 antibody [JB40-98].
|
![Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_20200303_ICC-IF_493_20030422_410.jpg "Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122")
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GTX01122 ICC/IF Image
ICC/IF analysis of PFA-fixed K562 cells using GTX01122 CD27 antibody [JB40-98].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
|
![Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_20200303_FACS_496_20030422_865.jpg "Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122")
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GTX01122 FACS Image
FACS analysis of Daudi cells using GTX01122 CD27 antibody [JB40-98].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100
|
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/middle/GTX01122/GTX01122_20200303_IHC-P_495_20030422_501.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
|
GTX01122 IHC-P Image
IHC-P analysis of rat spleen tissue section using GTX01122 CD27 antibody [JB40-98].
|
REFERENCE
There are currently no references for CD27 antibody [JB40-98] (GTX01122). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD27 antibody [JB40-98] (GTX01122). Be the first to share your experience with this product.
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_HM1119_20200317_WB_20032502_471.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_HM1119_20200814_WB_R_spleen_20082000_593.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_20200303_IHC-P_494_20030422_713.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_20200303_ICC-IF_493_20030422_410.jpg "Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_20200303_FACS_496_20030422_865.jpg "Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122")
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/small/GTX01122/GTX01122_20200303_IHC-P_495_20030422_501.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_HM1119_20200317_WB_20032502_471.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_HM1119_20200814_WB_R_spleen_20082000_593.jpg "Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122")
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_20200303_IHC-P_494_20030422_713.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_20200303_ICC-IF_493_20030422_410.jpg "Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122")
![Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_20200303_FACS_496_20030422_865.jpg "Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122")
![Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122](/upload/website/prouct_img/normal/GTX01122/GTX01122_20200303_IHC-P_495_20030422_501.jpg "Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122")
![Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363](/upload/website/prouct_img/small/GTX635363/GTX635363_20200217_IHC-Fr_M_competitor_20021701_920.jpg "Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363")
![Anti-CD8 antibody [LT8] used in IHC (Frozen sections) (IHC-Fr). GTX74773](/upload/website/prouct_img/small/GTX74773/GTX74773_2877_IHC-Fr_18121411_669.jpg "Anti-CD8 antibody [LT8] used in IHC (Frozen sections) (IHC-Fr). GTX74773")
