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CD27 antibody [JB40-98]

Recombinant antibody
Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122
Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122
Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122
Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122
Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122
Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122

Cat No. GTX01122

Host

Rabbit

Clonality

Monoclonal

Clone Name

JB40-98

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD27 molecule , CD27 , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122

GTX01122 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD27 antibody [JB40-98] (GTX01122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD27 antibody [JB40-98] used in Western Blot (WB). GTX01122

GTX01122 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD27 antibody [JB40-98] (GTX01122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122

GTX01122 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01122 CD27 antibody [JB40-98].

Anti-CD27 antibody [JB40-98] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01122

GTX01122 ICC/IF Image

ICC/IF analysis of PFA-fixed K562 cells using GTX01122 CD27 antibody [JB40-98].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-CD27 antibody [JB40-98] used in Flow cytometry (FACS). GTX01122

GTX01122 FACS Image

FACS analysis of Daudi cells using GTX01122 CD27 antibody [JB40-98].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-CD27 antibody [JB40-98] used in IHC (Paraffin sections) (IHC-P). GTX01122

GTX01122 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX01122 CD27 antibody [JB40-98].

REFERENCE

There are currently no references for CD27 antibody [JB40-98] (GTX01122). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD27 antibody [JB40-98] (GTX01122). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369