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CD27 antibody [LPFS2/1611]

Anti-CD27 antibody [LPFS2/1611] used in Flow cytometry (FACS). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in Protein Array (Protein Array). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479
Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479

Cat No. GTX34479

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LPFS2/1611

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($339)
See all CD27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
ELISA Assay dependent
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CD27 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD27 , CD27 molecule , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD27 antibody [LPFS2/1611] used in Flow cytometry (FACS). GTX34479

GTX34479 FACS Image

FACS analysis of Ramos cells using GTX34479 CD27 antibody [LPFS2/1611].
Blue : Primary antibody
Red : Isotype control

Anti-CD27 antibody [LPFS2/1611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34479

GTX34479 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX34479 CD27 antibody [LPFS2/1611].
Green : Primary antibody
Red : nucleus

Anti-CD27 antibody [LPFS2/1611] used in Protein Array (Protein Array). GTX34479

GTX34479 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27 Mouse Monoclonal Antibody (LPFS2/1611). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479

GTX34479 IHC-P Image

IHC-P analysis of human colon tissue using GTX34479 CD27 antibody [LPFS2/1611].

Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479

GTX34479 IHC-P Image

IHC-P analysis of human spleen tissue using GTX34479 CD27 antibody [LPFS2/1611].

Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479

GTX34479 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34479 CD27 antibody [LPFS2/1611].

Anti-CD27 antibody [LPFS2/1611] used in IHC (Paraffin sections) (IHC-P). GTX34479

GTX34479 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34479 CD27 antibody [LPFS2/1611].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339