GeneTex
United States (US)

CD27 antibody

GTX104406_39988_IHC_19011506_264.jpg
GTX104406_39988_WB_19011506_812.jpg

Cat No. GTX104406

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

K562
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC20393 antibody, S152 antibody, T14 antibody, TNFRSF7 antibody, Tp55 antibody, CD27 antibody, T-cell activation antigen CD27 antibody, CD27 antigen antibody, tumor necrosis factor receptor superfamily member 7 antibody, T cell activation antigen S152 antibody, CD27L receptor antibody, "tumor necrosis factor receptor superfamily, member 7 antibody", T cell activation antigen CD27 antibody, CD27 molecule antibody

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX104406_39988_IHC_19011506_264.jpg

GTX104406 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD27(GTX104406) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104406_39988_WB_19011506_812.jpg

GTX104406 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX104406 diluted at 1:10000

SDS
Glycerol.pdf
Thimerosal.pdf