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CD27 antibody

Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555
Anti-CD27 antibody used in Western Blot (WB). GTX55555
Anti-CD27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55555
Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555
Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555
Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555

Cat No. GTX55555

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD27 molecule , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555

GTX55555 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX55555 CD27 antibody.
Dilution : 1:100

Anti-CD27 antibody used in Western Blot (WB). GTX55555

GTX55555 WB Image

WB analysis of various sample lysates using GTX55555 CD27 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-CD27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55555

GTX55555 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX55555 CD27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555

GTX55555 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55555 CD27 antibody.
Dilution : 1:100

Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555

GTX55555 IHC-P Image

IHC-P analysis of mouse thymus tissue using GTX55555 CD27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD27 antibody used in IHC (Paraffin sections) (IHC-P). GTX55555

GTX55555 IHC-P Image

IHC-P analysis of rat thymus tissue using GTX55555 CD27 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339