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CD28 antibody, C-term

Anti-CD28 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81608
Anti-CD28 antibody, C-term used in Western Blot (WB). GTX81608

Cat. No. GTX81608

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
400 μl ($399)
See all CD28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD28 molecule , Tp44

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-CD28 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81608

GTX81608 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81608 CD28 antibody, C-term.
Green : CD28
Blue : DAPI

Anti-CD28 antibody, C-term used in Western Blot (WB). GTX81608

GTX81608 WB Image

WB analysis of MDA-MB231 cell lysate (35ug/lane) using GTX81608 CD28 antibody, C-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399