CD28 antibody [CD28.2] (FITC)

Cat. No. GTX80197

Host	Mouse
Clonality	Monoclonal
Clone Name	CD28.2
Isotype	IgG1
Application	FACS
Reactivity	Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package	100 test ($329)

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View product citations for antibody GTX80197 on CiteAb

PRODUCT

Summary

The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD28 molecule , Tp44

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Gene ID: 940 CD28
UniProt: P10747 CD28

Research Area

DATA IMAGES

GTX80197 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX80197 CD28 antibody [CD28.2] (FITC).
Solid line : Primary antibody
Dashed line : FITC mouse IgG1 isotype control
Antibody amount : 0.5 μg