GeneTex
United States (US)

CD28 antibody [CD28.2] (FITC)

CD28-antibody-CD282-FITC__GTX80197-1_18121409_639.gif

Cat No. GTX80197

Host Mouse
Clonality Monoclonal
Clone Name CD28.2
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, FACS, IP, Functional Assay
Reactivity Human, Primate
APPLICATION

Application Note

Recommended Starting Dilutions:
Functional: T cell costimulation
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
PROPERTIES

Form

Liquid

Buffer

The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Antigen Species

Human

Immunogen

DC28.1.3.3 hybridoma cells

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd28 Molecule,Tp44,Cd28

Background

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Entrez Gene: 2q33

Database

Research Area

DATA IMAGES
CD28-antibody-CD282-FITC__GTX80197-1_18121409_639.gif

GTX80197 FACS Image

Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified (GTX80196).