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United States (US)

CD28 antibody [E18] (Low endotoxin, azide free)

Cat. No. GTX42134

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 E18

Isotype

 IgG2b

Application

 FACS, Activation

Reactivity

 Mouse
Package
500 μg ($749)
See all CD28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1/50-1/100
Activation Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Activation
Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Cd28 Antigen , Cd28

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 749
Custom Conjugations