APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human CD28 Intracellular domain.
Predict Reactivity
Mouse, Rat, Rabbit, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human CD28. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD28 molecule , Tp44
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Database
Research Area
DATA IMAGES
![Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068](/templates/default/js/lightbox/images/loading.gif "Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068")
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GTX637068 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD28 antibody [HL1589] (GTX637068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637068 IHC-P Image
CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat spleen.CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637068 IHC-P Image
CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded dog spleen.CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637068 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD28 antibody [HL1589] (GTX637068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637068 ICC/IF Image
CD28 antibody [HL1589] detects CD28 protein at cell membrane by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD28 stained by CD28 antibody [HL1589] (GTX637068) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for CD28 antibody [HL1589] (GTX637068). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD28 antibody [HL1589] (GTX637068). Be the first to share your experience with this product.
![Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068](/upload/website/prouct_img/small/GTX637068/GTX637068_44767_20220812_WB_22081423_100.webp "Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068")
![Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068](/upload/website/prouct_img/small/GTX637068/GTX637068_44767_20230203_IHC-P_Cat_23020621_481.webp "Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068")
![Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068](/upload/website/prouct_img/small/GTX637068/GTX637068_44767_20230203_IHC-P_Dog_23020621_148.webp "Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068")
![Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068](/upload/website/prouct_img/small/GTX637068/GTX637068_44767_20240308_WB_treatment_PNGaseF_24031220_597.webp "Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068")
![Anti-CD28 antibody [HL1589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637068](/upload/website/prouct_img/small/GTX637068/GTX637068_44767_20221007_ICC_IF_22102723_658.webp "Anti-CD28 antibody [HL1589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637068")
![Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068](/upload/website/prouct_img/normal/GTX637068/GTX637068_44767_20220812_WB_22081423_100.webp "Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068")
![Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068](/upload/website/prouct_img/normal/GTX637068/GTX637068_44767_20230203_IHC-P_Cat_23020621_481.webp "Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068")
![Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068](/upload/website/prouct_img/normal/GTX637068/GTX637068_44767_20230203_IHC-P_Dog_23020621_148.webp "Anti-CD28 antibody [HL1589] used in IHC (Paraffin sections) (IHC-P). GTX637068")
![Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068](/upload/website/prouct_img/normal/GTX637068/GTX637068_44767_20240308_WB_treatment_PNGaseF_24031220_597.webp "Anti-CD28 antibody [HL1589] used in Western Blot (WB). GTX637068")
![Anti-CD28 antibody [HL1589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637068](/upload/website/prouct_img/normal/GTX637068/GTX637068_44767_20221007_ICC_IF_22102723_658.webp "Anti-CD28 antibody [HL1589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637068")