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CD28 antibody [YTH913.12] (Low endotoxin, azide free)

Cat. No. GTX42131

Reference 21

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YTH913.12

Isotype

 IgG2b

Application

 IHC-Fr, FACS, IP, Functional Assay, Activation

Reactivity

 Human
Package
100 μg ($399)
See all CD28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
Functional Assay Assay dependent
Activation Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood T-cells.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD28 molecule , Tp44

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

REFERENCE

Clone Reference

Aguilar-Flores C et al. Mediators Inflamm 2020; 2020:8065147 Association of Pathogenic Th17 Cells with the Disease Severity and Its Potential Implication for Biological Treatment Selection in Psoriasis Patients.
Svensson-Arvelund J et al. J Immunol 2015; 194 (4):1534-44 The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages.
Application : Activation
Reactivity : Human
Litjens NH et al. J Immunol 2012; 188 (3):1083-90 Identification of circulating human antigen-reactive CD4+ FOXP3+ natural regulatory T cells.
Application : Activation
Reactivity : Human
Pridgeon C et al. Clin Sci (Lond) 2011; 120 (12):515-24 Regulation of IL-17 in chronic inflammation in the human lung.
Application : Activation
Reactivity : Human
Mjosberg J et al. J Immunol 2009; 183 (1):759-69 systemic reduction of functionally suppressive cd4dimcd25highfoxp3+ tregs in human second trimester pregnancy is induced by progesterone and 17beta-*.
Reactivity : Human
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REVIEW

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Clone Reference
Aguilar-Flores C et al. Mediators Inflamm 2020; 2020:8065147 Association of Pathogenic Th17 Cells with the Disease Severity and Its Potential Implication for Biological Treatment Selection in Psoriasis Patients.
Svensson-Arvelund J et al. J Immunol 2015; 194 (4):1534-44 The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages.
Application : Activation
Reactivity : Human
Litjens NH et al. J Immunol 2012; 188 (3):1083-90 Identification of circulating human antigen-reactive CD4+ FOXP3+ natural regulatory T cells.
Application : Activation
Reactivity : Human
Pridgeon C et al. Clin Sci (Lond) 2011; 120 (12):515-24 Regulation of IL-17 in chronic inflammation in the human lung.
Application : Activation
Reactivity : Human
Mjosberg J et al. J Immunol 2009; 183 (1):759-69 systemic reduction of functionally suppressive cd4dimcd25highfoxp3+ tregs in human second trimester pregnancy is induced by progesterone and 17beta-*.
Reactivity : Human
Verkade MA et al. Nephrol Dial Transplant 2007; 22 (1):128-38 Functional impairment of monocyte-derived dendritic cells in patients with severe chronic kidney disease.
Reactivity : Human
Kropf P et al. Eur J Immunol 2007; 37 (4):935-45 Arginase activity mediates reversible T cell hyporesponsiveness in human pregnancy.
Gabdoulkhakova A et al. Immunogenetics 2007; 59 (2):135-43 High rate of mutation reporter gene inactivation during human T cell proliferation.
Application : Activation
Reactivity : Human
Goodier MR et al. Immunology 2004; 111 (4):384-90 CD28 is not directly involved in the response of human CD3- CD56+ natural killer cells to lipopolysaccharide: a role for T cells.
Battaglia A et al. Immunology 2003; 110 (3):304-12 Lymphocyte populations in human lymph nodes. Alterations in CD4+ CD25+ T regulatory cell phenotype and T-cell receptor Vbeta repertoire.
Application : Activation
Reactivity : Human
Blanco B et al. J Immunol 2003; 171 (2):1070-7 Induction of human T lymphocyte cytotoxicity and inhibition of tumor growth by tumor-specific diabody-based molecules secreted from gene-modified bystander cells.
Application : Activation
Reactivity : Human
Ponchel F et al. Blood 2002; 100 (13):968046 Dysregulated lymphocyte proliferation and differentiation in patients with rheumatoid arthritis.
Application : Activation
Reactivity : Human
Costa C et al. J Immunol 2002; 168 (8):3808-16 Human NK cell-mediated cytotoxicity triggered by CD86 and Gal alpha 1,3-Gal is inhibited in genetically modified porcine cells.
Chuang E et al. Immunity 2000; 13 (3):313-22 The CD28 and CTLA-4 receptors associate with the serine/threonine phosphatase PP2A.
Application : IP
Reactivity : Human
Wilson JL et al. J Immunol 1999; 163 (8):842950 NK cell triggering by the human costimulatory molecules CD80 and CD86.
Application : FACS
Reactivity : Human
Boulougouris G et al. J Immunol 1999; 163 (4):1809-16 IL-2-independent activation and proliferation in human T cells induced by CD28.
Application : FACS
Reactivity : Human
Galea-Lauri J et al. J Immunol 1999; 163 (1):62-70 Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells.
Kaga S et al. J Immunol 1998; 160 (9):833729 Activation of p21-CDC42/Rac-activated kinases by CD28 signaling: p21-activated kinase (PAK) and MEK kinase 1 (MEKK1) may mediate the interplay between CD3 and CD28 signals.
Application : Activation
Reactivity : Human
McLeod JD et al. J Immunol 1998; 160 (5):63068 Activation of human T cells with superantigen (staphylococcal enterotoxin B) and CD28 confers resistance to apoptosis via CD95.
Application : FACS
Reactivity : Human
Koulis A et al. Am J Pathol 1997; 151 (5):1353-60 Characterization of tumor-infiltrating T lymphocytes in B-cell lymphomas of mucosa-associated lymphoid tissue.
Application : IHC-Fr
Reactivity : Human
Pawelec G et al. Cell Immunol 1990; 129 (2):385-93 Human T cell clones with gamma/delta and alpha/beta receptors are differently stimulated by monoclonal antibodies to CD2.
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
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