GeneTex
United States (US)

CD28 (phospho Tyr218) antibody

GTX55086_20190131_WB_19013101_407.jpg

Cat No. GTX55086

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of CD28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd28 Molecule,Tp44,Cd28

Cellular Localization

Cell surface,Membrane

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES
GTX55086_20190131_WB_19013101_407.jpg

GTX55086 WB Image

WB analysis of colchicine-treated HeLa (A), Jurkat (B), colchicine-treated Raw264.7 (C), colchicine-treated H9C2 (D) whole cell lysates using GTX55086 CD28 (phospho Tyr218) antibody.