GeneTex
United States (US)

CD2AP antibody

Comparison
GTX106235_40142_20170825_WB_competitor_watermark_19011506_404.jpg
GTX106235_40142_WB_R_19011506_620.jpg
GTX106235_40142_WB_19011506_187.jpg
GTX106235_40464_20171222_WB_competitor_watermark_19011506_363.jpg
GTX106235_40464_WB_19011506_868.jpg
GTX106235_40142_IHC_M_19011506_723.jpg
GTX106235_40142_IFA_19011506_187.jpg
GTX106235_IHC_19011506_700.jpg

Cat No. GTX106235

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Rat2 , K562 , THP-1 , HL-60

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CMS antibody, DKFZp586H0519 antibody, CD2AP antibody, CD2-associated protein antibody, Cas ligand with multiple Src homology 3 (SH3) domains antibody, cas ligand with multiple SH3 domains antibody, adapter protein CMS antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , ruffle

Background

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106235_40142_20170825_WB_competitor_watermark_19011506_404.jpg

GTX106235 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX106235_40142_WB_R_19011506_620.jpg

GTX106235 WB Image

CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106235_40142_WB_19011506_187.jpg

GTX106235 WB Image

CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106235_40464_20171222_WB_competitor_watermark_19011506_363.jpg

GTX106235 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX106235_40464_WB_19011506_868.jpg

GTX106235 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX106235 diluted at 1:1000

GTX106235_40142_IHC_M_19011506_723.jpg

GTX106235 IHC-P Image

CD2AP antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD2AP antibody (GTX106235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106235_40142_IFA_19011506_187.jpg

GTX106235 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP(GTX106235) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX106235_IHC_19011506_700.jpg

GTX106235 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein(GTX106235) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169