APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A375 , Rat2 , A431 , HeLa , HepG2 , K562 , THP-1 , HL-60
Predict Reactivity
Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.17 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036504
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD2 associated protein , CMS
Cellular Localization
Cytoplasm , cytoskeleton , Cell projection , ruffle
Background
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106235 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX106235 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody (GTX106235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX106235 WB Image
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX106235 diluted at 1:1000
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GTX106235 WB Image
CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106235 IHC-P Image
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein(GTX106235) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106235 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX106235 IHC-P Image
CD2AP antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD2AP antibody (GTX106235) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106235 WB Image
CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106235 ICC/IF Image
CD2AP antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD2AP stained by CD2AP antibody (GTX106235) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX106235 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP(GTX106235) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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REFERENCE
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