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CD3 antibody [1F4]

Anti-CD3 antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX76493

Cat No. GTX76493

Host

Mouse

Clonality

Monoclonal

Clone Name

1F4

Isotype

IgM

Application

WB, IHC-P, IHC-Fr, FACS, IP

Reactivity

Rat
Package
100 μg ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1/10
IHC-Fr 1/10-1/25
FACS 1/10-1/25
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Not tested in other applications.

Specificity/Sensitivity

In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

F344 strain rat T cells stimulated with PMA (TPA) and calcium ionophore

Purification

Ammonium sulphate precipitation
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-CD3 antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX76493

GTX76493 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX76493 CD3 antibody [1F4].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 219