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CD3 antibody [GT0013]

Anti-CD3 antibody [GT0013] used in Flow cytometry (FACS). GTX628462
Anti-CD3 antibody [GT0013] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628462
Anti-CD3 antibody [GT0013] used in Flow cytometry (FACS). GTX628462

Cat. No. GTX628462

Host

Mouse

Clonality

Monoclonal

Clone Name

GT0013

Isotype

IgG2a

Application

ICC/IF, FACS

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CD3

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888036

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-CD3 antibody [GT0013] used in Flow cytometry (FACS). GTX628462

GTX628462 FACS Image

CD3 antibody [GT0013] detects CD3 protein by flow cytometry analysis. Sample: B6 / spleen Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300 Red: CD3 antibody [GT0013] (GTX628462) dilution: 1:50.

Anti-CD3 antibody [GT0013] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628462

GTX628462 ICC/IF Image

CD3 antibody [GT0013] detects CD3 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD3 protein stained by CD3 antibody [GT0013] (GTX628462) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-CD3 antibody [GT0013] used in Flow cytometry (FACS). GTX628462

GTX628462 FACS Image

CD3 antibody [GT0013] detects CD3 protein by flow cytometry analysis.
Sample: Jurkat cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Black: Isotype control (ab18449) dilution: 1:50.
Green: CD3 antibody [GT0013] (GTX628462) dilution: 1:50.

REFERENCE

REVIEW

CD3 antibody [GT0013] Cat. No. GTX628462

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Oct-2013
Score :
Application Tested : FACS
Sample Species : Ms
Sample : B6 / spleen cells
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300, Red: CD3 antibody [GT0013] (GTX628462)
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations