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CD3 antibody [Hit3a] (APC)

Anti-CD3 antibody [Hit3a] (APC) used in Flow cytometry (FACS). GTX01459-07

Cat. No. GTX01459-07

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Hit3a

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human, Chimpanzee
Package
100 test ($149)
See all CD3 products
PRODUCT

Summary

The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3e molecule , CD3g molecule , CD247 molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell membrane , Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD3 antibody [Hit3a] (APC) used in Flow cytometry (FACS). GTX01459-07

GTX01459-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01459-07 CD3 antibody [Hit3a] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

2012; Microparticle generation and leucocyte death in Shiga toxin-mediated HUS.
2012; Vav1 oncogenic mutation inhibits T cell receptor-induced calcium mobilization through inhibition of phospholipase Cγ1 activation.
2012; Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
2010; Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation.
2005; Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149