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CD3 antibody [Hit3a]

Cat. No. GTX01459

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Hit3a

Isotype

 IgG2a

Application

 ICC/IF, IHC-Fr, FACS, Activation, Neutralizing/Inhibition

Reactivity

 Human, Chimpanzee
Package
100 μg ($119)
See all CD3 products
PRODUCT

Summary

The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Activation Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3e molecule , CD3g molecule , CD247 molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell membrane , Membrane; Single-pass type I membrane protein

Database

Research Area

REFERENCE

Clone Reference

2012; Microparticle generation and leucocyte death in Shiga toxin-mediated HUS.
Application : ICC/IF
Reactivity : Human
2012; Vav1 oncogenic mutation inhibits T cell receptor-induced calcium mobilization through inhibition of phospholipase Cγ1 activation.
Application : Activation
Reactivity : Human
2012; Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.
Application : Activation
Reactivity : Human
2010; Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation.
Application : FACS
Reactivity : Human, Chimpanzee
2005; Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells.
Application : IHC-Fr
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 119