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CD3 antibody [OKT3]

Cat. No. GTX79906

Host

Mouse

Clonality

Monoclonal

Clone Name

OKT3

Isotype

IgG2a

Application

IHC-P, IHC-Fr, FACS, Activation, Depletion

Reactivity

Human, Chimpanzee
Package
100 μg ($399)
PRODUCT

Summary

The OKT3 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Activation Assay dependent
Depletion Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Commercially sensitive information

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11162614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3e molecule , CD3g molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell membrane

Database

Research Area

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399