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CD3 antibody [SK7] (APC-Cy7)

Anti-CD3 antibody [SK7] (APC-Cy7) used in Flow cytometry (FACS). GTX01460-15

Cat No. GTX01460-15

Host

Mouse

Clonality

Monoclonal

Clone Name

SK7

Isotype

IgG1

Application

FACS

Reactivity

Human, Chimpanzee
Package
100 test ($329)
PRODUCT

Summary

The SK7 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The SK7 antibody is a widely used phenotypic marker for human T cells. This antibody may induce T cell activation in the presence of monocytes. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Binding of clone SK7 can be blocked by an alternative Anti-Human CD3 clone, OKT3.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin-Cyanine7 (APC-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3d molecule , CD3e molecule , CD3g molecule , CD247 molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell membrane , Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD3 antibody [SK7] (APC-Cy7) used in Flow cytometry (FACS). GTX01460-15

GTX01460-15 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01460-15 CD3 antibody [SK7] (APC-Cy7).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.5 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 329