CD3 antibody [SK7] (PE)

Cat. No. GTX01460-08

Host	Mouse
Clonality	Monoclonal
Clone Name	SK7
Isotype	IgG1
Application	FACS
Reactivity	Human, Chimpanzee
Package	100 test ($349)

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View product citations for antibody GTX01460-08 on CiteAb

PRODUCT

Summary

The SK7 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The SK7 antibody is a widely used phenotypic marker for human T cells. This antibody may induce T cell activation in the presence of monocytes. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Binding of clone SK7 can be blocked by an alternative Anti-Human CD3 clone, OKT3.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3e molecule , CD3g molecule , CD247 molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cell membrane , Membrane; Single-pass type I membrane protein

Database

Gene ID: 915 CD3D
Gene ID: 916 CD3E
Gene ID: 917 CD3G
Gene ID: 919 CD247
UniProt: P04234 CD3D
UniProt: P09693 CD3E
UniProt: P20963 CD3G
UniProt: P07766 CD247

Research Area

DATA IMAGES

GTX01460-08 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01460-08 CD3 antibody [SK7] (PE).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)