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CD3 antibody [SP7]

Orthogonal
Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669
Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669

Cat. No. GTX16669

Reference 23

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SP7

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Pig
Package
500 μl ($499)
See all CD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:25-1:50
IHC-Fr Assay dependent
FACS Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Product Note

This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

A synthetic 13-mer peptide corresponding to aa 156-168 of the ε chain of human CD3 protein.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_425123

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3-DELTA , IMD19 , T3D

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669

GTX16669 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX16669 CD3 antibody [SP7].

Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669

GTX16669 IHC-P Image

Orthogonal

The data was published in the journal Cancers (Basel) in 2020.PMID: 32650570

REFERENCE

Application Reference

Hu X et al. J Immunother Cancer 2024; 12 (3) Inhibition of IL-25/IL-17RA improves immune-related adverse events of checkpoint inhibitors and reveals antitumor activity.
Morita TY et al. Nat Commun 2023; 14 (1) : 7490 CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockade.
Mascarau R et al. J Cell Biol 2023; 222 (5) Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells.
Application : IHC-P
Reactivity : Human
Jang B et al. Matrix Biol 2022; (Epub) Shed syndecan-2 enhances colon cancer progression by increasing cooperative angiogenesis in the tumor microenvironment.

Clone Reference

SS Popko et al. Health Sciences 2022; 2:17 - 22 The possibility of using anti-human monoclonal antibody CD3 as pan T-cell marker in guinea pigs
Application : IHC-P
Reactivity : Pig
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REVIEW

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Application Reference
Hu X et al. J Immunother Cancer 2024; 12 (3) Inhibition of IL-25/IL-17RA improves immune-related adverse events of checkpoint inhibitors and reveals antitumor activity.
Morita TY et al. Nat Commun 2023; 14 (1):7490 CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockade.
Mascarau R et al. J Cell Biol 2023; 222 (5) Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells.
Application : IHC-P
Reactivity : Human
Jang B et al. Matrix Biol 2022; (Epub) Shed syndecan-2 enhances colon cancer progression by increasing cooperative angiogenesis in the tumor microenvironment.
Shmuel-Galia L et al. Immunity 2021; 54 (6):1137-1153.e8 Dysbiosis exacerbates colitis by promoting ubiquitination and accumulation of the innate immune adaptor STING in myeloid cells.
Application : IHC-P
Reactivity : Mouse
He S et al. Inflammation 2021; 44 (3):835-845 Hydroxysafflor Yellow A Inhibits Staphylococcus aureus-Induced Mouse Endometrial Inflammation via TLR2-Mediated NF-kB and MAPK Pathway.
Sun Z et al. PLoS One 2021; 16 (2):e0247238 Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers.
Application : IHC-P
Reactivity : Human
Chan YL et al. Cancers (Basel) 2020; 12 (7) TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.
Heffer A et al. Transl Vis Sci Technol 2020; 9 (7):9 A Mouse Model of Proliferative Vitreoretinopathy Induced by Intravitreal Injection of Gas and RPE Cells.
Application : IHC-P
Reactivity : Mouse
Umar S et al. Cell Mol Immunol 2020; (Epub) IRAK4 inhibition: a promising strategy for treating RA joint inflammation and bone erosion.
Application : IHC-P
Reactivity : Mouse
Kirby L et al. Nat Commun 2019; 10 (1):3887 Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.
Application : IHC-Fr
Reactivity : Mouse
Chen PM et al. Biomaterials 2019; (Epub) Modulation of tumor microenvironment using a TLR-7/8 agonist-loaded nanoparticle system that exerts low-temperature hyperthermia and immunotherapy for in situ cancer vaccination.
Application : IHC-P
Reactivity : Mouse
Hong H et al. FASEB J 2019;:fj201900561R Up-regulation of syndecan-2 in proximal colon correlates with acute inflammation.
Van Raemdonck K et al. Cell Mol Life Sci 2019; CCL21/CCR7 signaling in macrophages promotes joint inflammation and Th17-mediated osteoclast formation in rheumatoid arthritis.
Application : IHC-P
Reactivity : Mouse
Y.Q.Yang et al. Phytomedicine 2019; (Epub) Tripterigium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.
Application : FACS
Reactivity : Human
SohjiNishina et al. Cellular and Molecular Gastroenterology and Hepatology 2018; (Epub) Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice.
Application : IHC-P
Reactivity : Human
Baird JR et al. Cancer Res 2018; (Epub) Evaluation of explant responses to STING ligands: personalized immunosurgical therapy for head and neck squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Lin YL et al. Proc Natl Acad Sci U S A 2016; (Epub) in vivo amelioration of endogenous antitumor autoantibodies via low-dose p4n through the lta4h/* a/baff pathway.
Application : IHC-P
Reactivity : Mouse
Jones MV et al. Acta Neuropathol Commun 2015; 3:28 Pathogenic aquaporin-4 reactive T cells are sufficient to induce mouse model of neuromyelitis optica.
Application : IHC-Fr
Reactivity : Mouse
Swindell WR et al. Sci Rep 2013; 3:1215 Robust shifts in S100a9 expression with aging: a novel mechanism for chronic inflammation.
Application : IHC-P
Reactivity : Mouse
Clone Reference
SS Popko et al. Health Sciences 2022; 2:17 - 22 The possibility of using anti-human monoclonal antibody CD3 as pan T-cell marker in guinea pigs
Application : IHC-P
Reactivity : Pig
Ester Arévalo Sureda et al. World J Gastroenterol. 2017; 23 (42):7531–7540 Induction of precocious intestinal maturation in T-cell deficient athymic neonatal rats
Application : IHC-P
Reactivity : Rat
Felts RL et al. Proc Natl Acad Sci U S A 2010; 107 (30):13336-41 3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells
Application : ICC/IF
SDS
Sodium Azide.pdf
Package List Price ($)
$ 499