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CD3 antibody [SP7]

Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669

Cat. No. GTX16669

Host

Rabbit

Clonality

Monoclonal

Clone Name

SP7

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Pig
Package
500 μl ($499)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:25-1:50
IHC-Fr Assay dependent
FACS Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.

Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Product Note

This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

A synthetic 13-mer peptide corresponding to aa 156-168 of the ε chain of human CD3 protein.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_425123

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3d molecule , CD3-DELTA , IMD19 , T3D

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-CD3 antibody [SP7] used in IHC (Paraffin sections) (IHC-P). GTX16669

GTX16669 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX16669 CD3 antibody [SP7].

REFERENCE

REVIEW

CD3 antibody [SP7] Cat. No. GTX16669

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancers (Basel) in 2020.PMID: 32650570
Application Reference
Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 499