Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:25-1:50 |
Assay dependent |
Assay dependent |
Note :
IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0
|
Not tested in other applications.
Product Note
This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
Form
Liquid
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Antigen Species
Human
Immunogen
A synthetic 13-mer peptide corresponding to aa 156-168 of the ε chain of human CD3 protein.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_425123
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CD3d molecule , CD3-DELTA , IMD19 , T3D
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Database
Research Area