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CD3 epsilon antibody [4E2]

Anti-CD3 epsilon antibody [4E2] used in Flow cytometry (FACS). GTX83278
Anti-CD3 epsilon antibody [4E2] used in Western Blot (WB). GTX83278

Cat. No. GTX83278

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E2

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of CD3E expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [4E2] used in Flow cytometry (FACS). GTX83278

GTX83278 FACS Image

FACS analysis of Jurkat cells using GTX83278 CD3 epsilon antibody [4E2].
Green : CD3 epsilon
Purple : negative control

Anti-CD3 epsilon antibody [4E2] used in Western Blot (WB). GTX83278

GTX83278 WB Image

WB analysis of Jurkat cell lysate using GTX83278 CD3 epsilon antibody [4E2].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399