CD3 epsilon antibody [C3e/1931]

Cat. No. GTX17861

Host	Mouse
Clonality	Monoclonal
Clone Name	C3e/1931
Isotype	IgG1
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX17861 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
ELISA	2-4μg/ml (for coating)
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant human CD3e protein fragment (around aa 23-119) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Gene ID: 916 CD3E
UniProt: P07766 CD3E

Research Area

DATA IMAGES

GTX17861 FACS Image

FACS analysis of Jurkat cells using GTX17861 CD3 epsilon antibody [C3e/1931].
Blue : Primary antibody
Red : Isotype control

GTX17861 WB Image

WB analysis of Jurkat cell lysate using GTX17861 CD3 epsilon antibody [C3e/1931].

GTX17861 IHC-P Image

IHC-P analysis of human spleen tissue using GTX17861 CD3 epsilon antibody [C3e/1931].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty