APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 2-4μg/ml (for coating) |
| Assay dependent |
|
Note :
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CD3e fragment (around aa 23-119) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon
Cellular Localization
Membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865](/templates/default/js/lightbox/images/loading.gif "Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865")
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GTX17865 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD3e Mouse Monoclonal Antibody (C3e/2479). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX17865 WB Image
WB analysis of Jurkat cell lysate using GTX17865 CD3 epsilon antibody [C3e/2479].
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REFERENCE
There are currently no references for CD3 epsilon antibody [C3e/2479] (GTX17865). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD3 epsilon antibody [C3e/2479] (GTX17865). Be the first to share your experience with this product.
![Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865](/upload/website/prouct_img/small/GTX17865/GTX17865_20200115_Protein-Array_1999_w_23060620_487.webp "Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865")
![Anti-CD3 epsilon antibody [C3e/2479] used in Western Blot (WB). GTX17865](/upload/website/prouct_img/small/GTX17865/GTX17865_20200115_WB_1459_w_23060620_362.webp "Anti-CD3 epsilon antibody [C3e/2479] used in Western Blot (WB). GTX17865")
![Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865](/upload/website/prouct_img/normal/GTX17865/GTX17865_20200115_Protein-Array_1999_w_23060620_487.webp "Anti-CD3 epsilon antibody [C3e/2479] used in Protein Array (Protein Array). GTX17865")
![Anti-CD3 epsilon antibody [C3e/2479] used in Western Blot (WB). GTX17865](/upload/website/prouct_img/normal/GTX17865/GTX17865_20200115_WB_1459_w_23060620_362.webp "Anti-CD3 epsilon antibody [C3e/2479] used in Western Blot (WB). GTX17865")