APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2 μg/ml |
1-2 μg/ml |
2-4 μg/ml (for coating) |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
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Not tested in other applications.
Calculated MW
Observed MW
20-25 kDa.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CD3e fragment (around aa 23-119)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3e molecule , IMD18 , T3E , TCRE
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX02605 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02605 CD3 epsilon antibody [C3e/3125R].
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GTX02605 WB Image
WB analysis of MOLT-4 whole cell lysate using GTX02605 CD3 epsilon antibody [C3e/3125R].
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REFERENCE
There are currently no references for CD3 epsilon antibody [C3e/3125R] (GTX02605). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD3 epsilon antibody [C3e/3125R] (GTX02605). Be the first to share your experience with this product.