CD3 epsilon antibody [C3e/3125R]

Cat No. GTX02605

Host	Rabbit
Clonality	Monoclonal
Clone Name	C3e/3125R
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human
Package	100 μg ($429)

See all CD3 epsilon products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2 μg/ml
IHC-P	1-2 μg/ml
ELISA	2-4 μg/ml (for coating)
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Observed MW

20-25 kDa.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CD3e fragment (around aa 23-119)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Gene ID: 916 CD3E
UniProt: P07766 CD3E

Research Area

DATA IMAGES

GTX02605 WB Image

WB analysis of MOLT-4 whole cell lysate using GTX02605 CD3 epsilon antibody [C3e/3125R].

GTX02605 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02605 CD3 epsilon antibody [C3e/3125R].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 429

Qty

CD3 epsilon antibody [C3e/3125R]

Cat No. GTX02605

Host

Clonality

Clone Name

Isotype

Application

Reactivity