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CD3 epsilon antibody [CA-3]

Anti-CD3 epsilon antibody [CA-3] used in Flow cytometry (FACS). GTX10869

Cat. No. GTX10869

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CA-3

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS

Reactivity

 Human
Package
100 μg ($449)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 0.5-1μg/ml
IHC-Fr 1μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

23.15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 1.2% Sodium acetate, 2mg BSA

Preservative

0.01mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human thymocytes followed by Sezary T cells.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [CA-3] used in Flow cytometry (FACS). GTX10869

GTX10869 FACS Image

FACS analysis of human PBMC cells using GTX10869 CD3 epsilon antibody [CA-3].
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449