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CD3 epsilon antibody [CD3-12]

Anti-CD3 epsilon antibody [CD3-12] used in Flow cytometry (FACS). GTX42110
Anti-CD3 epsilon antibody [CD3-12] used in Western Blot (WB). GTX42110
Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX42110

Cat No. GTX42110

Host

Rat

Clonality

Monoclonal

Clone Name

CD3-12

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rabbit, Bovine, Cat, Dog, Chicken, Pig, Alpaca, Amazon Parrot, Bullfrog, Cynomolgus monkey, Duck, Great horned owl (Bubo virginianus), Harbour Porpoise, Horse, Koala, Raccoon, Rhesus Monkey, Sea Lion, Spotted Hyena, Xenopus
Package
25 μg ($199)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/100
IHC-Fr 1/100
FACS 1/50-1/100

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.

FACS
Membrane permeabilization is required for this application.

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC)

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11178619

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [CD3-12] used in Flow cytometry (FACS). GTX42110

GTX42110 FACS Image

FACS analysis of red cell lysed human peripheral blood gated on leukocytes using GTX42110 CD3 antibody [CD3-12].
Figure A : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat IgG1 isotype control.
Figure B : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat anti Human CD3 (GTX42110).

Anti-CD3 epsilon antibody [CD3-12] used in Western Blot (WB). GTX42110

GTX42110 WB Image

WB analysis of Jurkat cell lysate using GTX42110 CD3 antibody [CD3-12].

Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX42110

GTX42110 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX42110 CD3 antibody. Heat mediated antigen retrieval using EDTA buffer pH9.0.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 199