CD3 epsilon antibody [UCHT1] (APC)

Cat. No. GTX00598-07

Host	Mouse
Clonality	Monoclonal
Clone Name	UCHT1
Isotype	IgG1
Application	FACS
Reactivity	Human, Chimpanzee
Package	100 test ($359)

See all CD3 epsilon products

View product citations for antibody GTX00598-07 on CiteAb

PRODUCT

Summary

The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody reacts with the epsilon chain of the CD3 complex.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

human thymocytes followed by Sezary T cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Gene ID: 916 CD3E
UniProt: P07766 CD3E

Research Area

DATA IMAGES

GTX00598-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00598-07 CD3 epsilon antibody [UCHT1] (APC).
Solid line : Primary antibody
Dashed line : APC mouse IgG1 isotype control
Antibody amount : 0.25 μg