PRODUCT
Summary
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with the epsilon chain of the CD3 complex.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
human thymocytes followed by Sezary T cells
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Biotin
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3e molecule , IMD18 , T3E , TCRE
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02](/templates/default/js/lightbox/images/loading.gif "Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02")
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GTX00598-02 FACS Image
FACS analysis of human peripheral blood lymphocytes using GTX00598-02 CD3 epsilon antibody [UCHT1] (Biotin).
Solid line : Primary antibody
Dashed line : Biotin mouse IgG1 isotype control
Antibody amount : 0.25 μg
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REFERENCE
REVIEW
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![Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02](/upload/website/prouct_img/small/GTX00598-02/GTX00598-02_20201020_FACS_15_w_23053121_339.webp "Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02")
![Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02](/upload/website/prouct_img/normal/GTX00598-02/GTX00598-02_20201020_FACS_15_w_23053121_339.webp "Anti-CD3 epsilon antibody [UCHT1] (Biotin) used in Flow cytometry (FACS). GTX00598-02")