GeneTex
  • Country / Location Selection

United States (US)

CD3 epsilon antibody [UCHT1] (FITC)

Anti-CD3 epsilon antibody [UCHT1] (FITC) used in Flow cytometry (FACS). GTX76167

Cat No. GTX76167

Host

Mouse

Clonality

Monoclonal

Clone Name

UCHT1

Isotype

IgG1

Application

FACS

Reactivity

Human
Package
100 test ($329)
PRODUCT

Summary

The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody reacts with the epsilon chain of the CD3 complex.

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

human thymocytes followed by Sezary T cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [UCHT1] (FITC) used in Flow cytometry (FACS). GTX76167

GTX76167 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX76167 CD3 epsilon antibody [UCHT1] (FITC).
Solid line : Primary antibody
Dashed line : FITC mouse IgG1 isotype control.
Antibody amount : 1 μg

REFERENCE

REVIEW

There are currently no reviews for CD3 epsilon antibody [UCHT1] (FITC) (GTX76167). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329