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CD3 epsilon antibody [UCHT1] (PE-Cy7)

Anti-CD3 epsilon antibody [UCHT1] (PE-Cy7) used in Flow cytometry (FACS). GTX00598-10

Cat No. GTX00598-10

Host

Mouse

Clonality

Monoclonal

Clone Name

UCHT1

Isotype

IgG1

Application

FACS

Reactivity

Human, Chimpanzee
Package
100 test ($319)
PRODUCT

Summary

The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody reacts with the epsilon chain of the CD3 complex.

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml

Antigen Species

Human

Immunogen

human thymocytes followed by Sezary T cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [UCHT1] (PE-Cy7) used in Flow cytometry (FACS). GTX00598-10

GTX00598-10 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00598-10 CD3 epsilon antibody [UCHT1] (PE-Cy7).
Solid line : Primary antibody
Dashed line : PE-Cy7 mouse IgG1 isotype control
Antibody amount : 0.5 μg

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319