CD3 gamma antibody [C1C3]

Name: CD3 gamma antibody [C1C3]
Brand: GeneTex
SKU: GTX111878
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-CD3 gamma antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111878

Anti-CD3 gamma antibody [C1C3] used in Flow cytometry (FACS). GTX111878

Anti-CD3 gamma antibody [C1C3] used in Western Blot (WB). GTX111878

Cat. No. GTX111878

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CD3 gamma products

Datasheet
Document

View product citations for antibody GTX111878 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD3 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3g molecule , CD3-GAMMA , IMD17 , T3G

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq]

Database

Gene ID: 917 CD3G
UniProt: P09693 CD3G

Research Area

DATA IMAGES

GTX111878 IHC-P Image

CD3 gamma antibody [C1C3] detects CD3 gamma protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human PANC1 xenograft.
CD3 gamma antibody [C1C3] (GTX111878) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111878 FACS Image

CD3 gamma antibody [C1C3] (GTX111878) detects CD3 gamma protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD3 gamma antibody [C1C3] (GTX111878) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX111878 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX111878 diluted at 1:3000

REFERENCE

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REVIEW