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CD3 zeta antibody [4B10]

Anti-CD3 zeta antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX83004
Anti-CD3 zeta antibody [4B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83004
Anti-CD3 zeta antibody [4B10] used in Flow cytometry (FACS). GTX83004
Anti-CD3 zeta antibody [4B10] used in Western Blot (WB). GTX83004

Cat. No. GTX83004

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CD3 zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD247 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11169317

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD247 molecule , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 zeta antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX83004

GTX83004 IHC-P Image

IHC-P analysis of human Thymus tissue using GTX83004 CD3 zeta antibody [4B10].

Anti-CD3 zeta antibody [4B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83004

GTX83004 ICC/IF Image

ICC/IF analysis of K562 cells using GTX83004 CD3 zeta antibody [4B10].
Green : CD3 zeta
Blue: DRAQ5 fluorescent DNA dye

Anti-CD3 zeta antibody [4B10] used in Flow cytometry (FACS). GTX83004

GTX83004 FACS Image

FACS analysis of Jurkat cells using GTX83004 CD3 zeta antibody [4B10].
Green : CD3 zeta
Purple : negative control

Anti-CD3 zeta antibody [4B10] used in Western Blot (WB). GTX83004

GTX83004 WB Image

WB analysis of CD3 zeta(AA: 52-164)-hIgGFc transfected HEK293 cell lysate using GTX83004 CD3 zeta antibody [4B10].

REFERENCE

Application Reference

Kamakura D et al. Cancer Immunol Immunother 2020; Mechanism of action of a T cell-dependent bispecific antibody as a breakthrough immunotherapy against refractory colorectal cancer with an oncogenic mutation.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399