APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
CD247 transfected 293T cell
Predict Reactivity
Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human CD3 zeta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949925
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD247 molecule , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ
Cellular Localization
Membrane
Background
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479](/templates/default/js/lightbox/images/loading.gif "Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479")
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GTX108479 WB Image
Western Blot analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody.
A: Non-transfected lysate.
B: CD247 transfected lysate.
12% SDS PAGE
GTX108479 diluted at 1:500
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REFERENCE
There are currently no references for CD3 zeta antibody [C1C3] (GTX108479). Be the first to share your publications with this product.
REVIEW
CD3 zeta antibody [C1C3]
Cat. No. GTX108479
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 11-Mar-2024
Application Tested :
WB
Sample Species :
Hu
Sample :
Human αβT and Human γδT cells
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
![Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479](/upload/website/prouct_img/small/GTX108479/GTX108479_WB_w_23060120_422.webp "Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479")
![Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479](/upload/website/prouct_img/normal/GTX108479/GTX108479_WB_w_23060120_422.webp "Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479")
![Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479](/upload/website/support/rewardsprogram/normal/genetex_GTX108479_WB_1_20240311_24031100_702.webp "Anti-CD3 zeta antibody [C1C3] used in Western Blot (WB). GTX108479")