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CD3 zeta (phospho Tyr72) antibody [EM-26]

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Western Blot (WB). GTX00521
Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00521

Cat No. GTX00521

Host

Mouse

Clonality

Monoclonal

Clone Name

EM-26

Isotype

IgG2b

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD247 , CD247 molecule , CD3H , CD3Q , CD3Z , CD3ZETA , IMD25 , T3Z , TCRZ , CD3 zeta , CD3-ζ

Cellular Localization

Membrane

Background

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Western Blot (WB). GTX00521

GTX00521 WB Image

WB analysis of phosphorylated particular human CD3 zeta mutants using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26] and CD3 zeta (phosphoTyr153) [EM-17]. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00521

GTX00521 ICC/IF Image

ICC/IF analysis of immunological synapse formed between the lymph node naive T cells (from AND TCR transgenic mice) and DCEK cells loaded with MCC peptide using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26].
Green : total CD3 zeta
Blue : GTX00521
Red : Actin

REFERENCE

There are currently no references for CD3 zeta (phospho Tyr72) antibody [EM-26] (GTX00521). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD3 zeta (phospho Tyr72) antibody [EM-26] (GTX00521). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319