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CD3 zeta (phospho Tyr72) antibody [EM-26]

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Western Blot (WB). GTX00521
Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00521

Cat. No. GTX00521

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EM-26

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all CD3 zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD247 , CD247 molecule , CD3H , CD3Q , CD3Z , CD3ZETA , IMD25 , T3Z , TCRZ , CD3 zeta , CD3-ζ

Cellular Localization

Membrane

Background

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Western Blot (WB). GTX00521

GTX00521 WB Image

WB analysis of phosphorylated particular human CD3 zeta mutants using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26] and CD3 zeta (phosphoTyr153) [EM-17]. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

Anti-CD3 zeta (phospho Tyr72) antibody [EM-26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00521

GTX00521 ICC/IF Image

ICC/IF analysis of immunological synapse formed between the lymph node naive T cells (from AND TCR transgenic mice) and DCEK cells loaded with MCC peptide using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26].
Green : total CD3 zeta
Blue : GTX00521
Red : Actin

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399