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CD30 antibody [Ki-1/1747R]

Anti-CD30 antibody [Ki-1/1747R] used in IHC (Paraffin sections) (IHC-P). GTX34488

Cat No. GTX34488

Host Rabbit
Clonality Monoclonal
Clone Name Ki-1/1747R
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human full-length TNFRSF8 protein

Purification

Ab purified by Protein A Column

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Superfamily Member 8 , Cd30 , D1S166E , Ki-1 , Tnfrsf8

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-CD30 antibody [Ki-1/1747R] used in IHC (Paraffin sections) (IHC-P). GTX34488

GTX34488 IHC-P Image

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD30 Recombinant Rabbit Monoclonal Antibody (Ki-1/1747R).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429