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CD30 antibody

Anti-CD30 antibody used in Immunoprecipitation (IP). GTX66610
Anti-CD30 antibody used in Western Blot (WB). GTX66610

Cat. No. GTX66610

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IP 1:10-1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 500-600 of Human CD30.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 8 , CD30 , D1S166E , Ki-1

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 943 TNFRSF8
  • UniProt: P28908 TNFRSF8

Research Area

DATA IMAGES

Anti-CD30 antibody used in Immunoprecipitation (IP). GTX66610

GTX66610 IP Image

IP analysis of rat testis tissue lysate using GTX66610 CD30 antibody (Lane 2 and 3) and nonspecific IgG control antibody (Lane 4 and 5) compared to the input control (Lane 1).
Upward arrow : supernatant
Downward arrow : Immunoprecipitants

Anti-CD30 antibody used in Western Blot (WB). GTX66610

GTX66610 WB Image

WB analysis of various samples using GTX66610 CD30 antibody.
Lane1 : HCT116 whole cell lysate(40ug)
Lane2 : MCF-7 whole cell lysate(40ug)
Lane3 : CT26 whole cell lysate(40ug)
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399