CD31 antibody [2H8] (Azide free)

Name: CD31 antibody [2H8] (Azide free)
Brand: GeneTex
SKU: GTX74943
Price: 349.00 USD
Rating: 5 (1 reviews)

Cat No. GTX74943

Reference 4

Host	Armenian Hamster
Clonality	Monoclonal
Clone Name	2H8
Isotype	IgG
Application	ICC/IF, IHC-Wm, FACS, IHC, Neutralizing/Inhibition
Reactivity	Mouse
Package	250 μg ($349)

See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	Assay dependent
IHC-Wm	Assay dependent
FACS	0.1μg/ml
IHC	Assay dependent
Neutralizing/Inhibition	Assay dependent
Note : FACS Use 5μl of the suggested working dilution to label 10⁶ cells or 100μl whole blood. Neutralizing/Inhibition Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994).

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

D10.G4.1 cells (Kaye et al. 1984).

Purification

Purified by Caprylic Acid precipitation

Conjugation

Unconjugated

RRID

AB_378305

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet/endothelial cell adhesion molecule 1 , C85791 , Cd31 , PECAM-1 , Pecam

Cellular Localization

Cell membrane , Cell junction

Database

Gene ID: 18613 Pecam1
UniProt: Q08481 Pecam1

Research Area

REFERENCE

Application Reference

Song E et al. Nature 2020; 577 (7792) : 689-694 VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours.

Application : IHC-Wm

Reactivity : Mouse

Kilarski WW et al. Nat Commun 2019; 10 (1) : 2895 Inherent biomechanical traits enable infective filariae to disseminate through collecting lymphatic vessels.

Application : IHC-Wm

Reactivity : Mouse

Bradley W Richmond et al. Nat Commun 2016; 7 (0) : 11240 Airway bacteria drive a progressive COPD-like phenotype in mice with polymeric immunoglobulin receptor deficiency.

Application : IHC-Wm

Reactivity : Mouse

Kilarski WW et al. PLoS One 2013; 8 (2) : e57135 Intravital immunofluorescence for visualizing the microcirculatory and immune microenvironments in the mouse ear dermis.

Application : IHC

Reactivity : Mouse

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