GeneTex
  • Country / Location Selection

United States (US)

CD31 antibody [2H8] (Azide free)

Cat No. GTX74943

Host

Armenian Hamster

Clonality

Monoclonal

Clone Name

2H8

Isotype

IgG

Application

ICC/IF, IHC-Wm, FACS, IHC, Neutralizing/Inhibition

Reactivity

Mouse
Package
250 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-Wm Assay dependent
FACS 0.1μg/ml
IHC Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 5μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Neutralizing/Inhibition
Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994).

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

D10.G4.1 cells (Kaye et al. 1984).

Purification

Purified by Caprylic Acid precipitation

Conjugation

Unconjugated

RRID

AB_378305

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet/endothelial cell adhesion molecule 1 , C85791 , Cd31 , PECAM-1 , Pecam

Cellular Localization

Cell membrane , Cell junction

Database

Research Area

REFERENCE

REVIEW

CD31 antibody [2H8] (Azide free) Cat No. GTX74943

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 27046438
SDS
PBS.pdf
Package List Price ($)
$ 349