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CD31 antibody [C31.3]

Anti-CD31 antibody [C31.3] used in IHC (Paraffin sections) (IHC-P). GTX34490
Anti-CD31 antibody [C31.3] used in IHC (Paraffin sections) (IHC-P). GTX34490
Anti-CD31 antibody [C31.3] used in Western Blot (WB). GTX34490
Anti-CD31 antibody [C31.3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34490
Anti-CD31 antibody [C31.3] used in Flow cytometry (FACS). GTX34490

Cat. No. GTX34490

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C31.3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat
Package
100 μg ($529)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CD31 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD31 , CD31/EndoCAM , GPIIA' , PECA1 , PECAM1 , endoCAM , platelet and endothelial cell adhesion molecule 1

Cellular Localization

Cell membrane,Membrane raft,Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [C31.3] used in IHC (Paraffin sections) (IHC-P). GTX34490

GTX34490 IHC-P Image

IHC-P analysis of human angiosarcoma tissue using GTX34490 CD31 antibody [C31.3].

Anti-CD31 antibody [C31.3] used in IHC (Paraffin sections) (IHC-P). GTX34490

GTX34490 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34490 CD31 antibody [C31.3].

Anti-CD31 antibody [C31.3] used in Western Blot (WB). GTX34490

GTX34490 WB Image

WB analysis of Jurkat cell lysate using GTX34490 CD31 antibody [C31.3].

Anti-CD31 antibody [C31.3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34490

GTX34490 ICC/IF Image

ICC/IF analysis of PFA-fixed Jurkat cells using GTX34490 CD31 antibody [C31.3].
Green : Primary antibody
Red : nucleus

Anti-CD31 antibody [C31.3] used in Flow cytometry (FACS). GTX34490

GTX34490 FACS Image

FACS analysis of Jurkat cells using GTX34490 CD31 antibody [C31.3].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529