GeneTex
United States (US)

CD31 antibody [GT0010]

GTX632645_41190_FACS_18121411_687.jpg
GTX632645_41190_20151102_IHC-P_R_18121411_560.jpg
GTX632645_41190_20151102_IHC-P_R_2_18121411_807.jpg

Cat No. GTX632645

Host Hamster
Clonality Monoclonal
Clone Name GT0010
Isotype IgG
Application IHC-P, FACS
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Raw264.7
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CD31

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet And Endothelial Cell Adhesion Molecule 1,Cd31,Cd31/Endocam,Gpiia',Peca1,Pecam-1,Endocam,Pecam1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Database

Research Area

DATA IMAGES
GTX632645_41190_FACS_18121411_687.jpg

GTX632645 FACS Image

CD31 antibody detects CD31 protein by flow cytometry analysis.
Sample: Raw264.7 cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Black: Isotype control (GTX25739) dilution: 1:100.
Green: CD31 antibody (GTX632645) dilution: 1:100.

GTX632645_41190_20151102_IHC-P_R_18121411_560.jpg

GTX632645 IHC-P Image

CD31 antibody [GT0010] detects CD31 protein at cell membrane in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
CD31 antibody [GT0010] (GTX632645) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632645_41190_20151102_IHC-P_R_2_18121411_807.jpg

GTX632645 IHC-P Image

CD31 antibody [GT0010] detects CD31 protein at cell membrane in rat lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
CD31 antibody [GT0010] (GTX632645) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169