APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:50-1:500 |
3-5 μg/10⁶ cells |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human PECAM-1
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA'' , PECA1 , PECAM-1 , endoCAM
Cellular Localization
Cell membrane,Cell junction
Background
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
|
GTX15842 ICC/IF Image
ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4ºC
|
|
GTX15842 FACS Image
FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
|
|
GTX15842 FACS Image
FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
|
REFERENCE
There are currently no references for CD31 antibody [HEC7] (GTX15842). Be the first to share your publications with this product.
REVIEW
CD31 antibody [HEC7]
Cat. No. GTX15842
Rating
|
|
( Average 5 based on 1 users reviews)
|
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
|
|
( Average 5 based on 1 users reviews)
|
Date :
Anonymous submitted on 02-Sep-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
Human umbilical vein endothelial cells
Fixation :
4% PFA in PBS, RT, 10 minutes
Permeabilization /Antigen retrieval :
None
Primary Antibody :
1:200, 20°C, 1Hr
Figure Note :
Human umbilical vein endothelial cells at 40x magnification