GeneTex
United States (US)

CD31 antibody [HEC7]

GTX15842_124_FACS_19011519_267.jpg
GTX15842_1994_FACS_19011519_635.jpg
GTX15842_342_ICC-IF_19011519_379.jpg

Cat No. GTX15842

Host Mouse
Clonality Monoclonal
Clone Name HEC7
Isotype IgG2a
Application WB, ICC/IF, FACS, IP, ELISA, IHC, Neut
Reactivity Human, Rat, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:50-1:500
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PECAM-1

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet And Endothelial Cell Adhesion Molecule 1,Cd31,Cd31/Endocam,Gpiia',Peca1,Pecam-1,Endocam,Pecam1

Cellular Localization

Cell junction,Cell membrane,Membrane raft

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES
GTX15842_124_FACS_19011519_267.jpg

GTX15842 FACS Image

FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15842_1994_FACS_19011519_635.jpg

GTX15842 FACS Image

FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15842_342_ICC-IF_19011519_379.jpg

GTX15842 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4°C

SDS
PBS.pdf
Package List Price ($)
$ 319