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CD31 antibody [HEC7]

Anti-CD31 antibody [HEC7] used in Flow cytometry (FACS). GTX15842
Anti-CD31 antibody [HEC7] used in Flow cytometry (FACS). GTX15842
Anti-CD31 antibody [HEC7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15842

Cat No. GTX15842

Host

Mouse

Clonality

Monoclonal

Clone Name

HEC7

Isotype

IgG2a

Application

WB, ICC/IF, FACS, IP, ELISA, IHC, Neutralizing/Inhibition

Reactivity

Human, Rat, Bovine
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:50-1:500
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PECAM-1

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA'' , PECA1 , PECAM-1 , endoCAM

Cellular Localization

Cell membrane,Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [HEC7] used in Flow cytometry (FACS). GTX15842

GTX15842 FACS Image

FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-CD31 antibody [HEC7] used in Flow cytometry (FACS). GTX15842

GTX15842 FACS Image

FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-CD31 antibody [HEC7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15842

GTX15842 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

REFERENCE

There are currently no references for CD31 antibody [HEC7] (GTX15842). Be the first to share your publications with this product.

REVIEW

CD31 antibody [HEC7] Cat No. GTX15842

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Human umbilical vein endothelial cells
Fixation : 4% PFA in PBS, RT, 10 minutes
Permeabilization /Antigen retrieval : None
Primary Antibody : 1:200, 20°C, 1Hr
Figure Note : Human umbilical vein endothelial cells at 40x magnification
SDS
PBS.pdf