*Optimal dilutions/concentrations should be determined by the researcher.
|1-2ug/ml for 30 minutes at RT
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Membrane preparation of a spleen from a patient with hairy cell leukemia
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Platelet And Endothelial Cell Adhesion Molecule 1 , Cd31 , Cd31/Endocam , Gpiia' , Peca1 , Pecam-1 , Endocam , Pecam1
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
GTX34495 IHC-P Image
Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31 Monoclonal Antibody (JC/70A)
GTX34495 FACS Image
Flow Cytometry of human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31 Monoclonal Antibody (JC/70A).
GTX34495 IHC-P Image
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD31 Monoclonal Antibody (JC/70A)