Host |
Rat |
---|---|
Clonality |
Monoclonal |
Clone Name |
MEC7.46 |
Isotype |
IgG1 |
Application |
ICC/IF, IHC-P, IHC-Fr, FACS, IP |
Reactivity |
Mouse |
Package |
100 μg ($399)
|
Application | Recommended Dilution |
---|---|
ICC/IF | Assay dependent |
IHC-P | Assay dependent |
IHC-Fr | Assay dependent |
FACS | Assay dependent |
IP | Assay dependent |
Note : IHC-PSerial paraffin-embedded 4 μm sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium. IHC-Fr Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. FACS No fixation or permeabilization needed. Epitope is sensitive to trypsin! IP Lysate of 35S-Methionine labeled 5HV cells was used. |
GTX54379 IHC-Fr ImageThe data was published in the journal Sci Rep in 2018.PMID: 29463847 |
Package | List Price ($) |
---|---|
$ 399 | |