GeneTex
United States (US)

CD31 antibody [N3C2], Internal

GTX110602_40037_20171201_IHC-P_1_19011507_754.jpg
GTX110602_40037_20160505_WB_H_19011507_171.jpg
GTX110602_40037_IHC_19011507_445.jpg

Cat No. GTX110602

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HUVEC
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD31. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet And Endothelial Cell Adhesion Molecule 1,Cd31,Cd31/Endocam,Gpiia',Peca1,Pecam-1,Endocam,Pecam1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Database

Research Area

DATA IMAGES
GTX110602_40037_20171201_IHC-P_1_19011507_754.jpg

GTX110602 IHC-P Image

CD31 antibody [N3C2], Internal detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
CD31 stained by CD31 antibody [N3C2], Internal (GTX110602) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110602_40037_20160505_WB_H_19011507_171.jpg

GTX110602 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD31 antibody [N3C2], Internal (GTX110602) diluted at 1:10000.

GTX110602_40037_IHC_19011507_445.jpg

GTX110602 IHC-P Image

CD31 antibody detects CD31 protein at cell membrane of endothelial cells in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD31 antibody (GTX110602) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169