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CD31 antibody [SPM122]

Anti-CD31 antibody [SPM122] used in IHC (Paraffin sections) (IHC-P). GTX34492

Cat No. GTX34492

Host Mouse
Clonality Monoclonal
Clone Name SPM122
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Rabbit, Cynomolgus monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Specificity/Sensitivity

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Membrane preparation of a spleen from a patient with hairy cell leukemia

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet And Endothelial Cell Adhesion Molecule 1,Cd31,Cd31/Endocam,Gpiia',Peca1,Pecam-1,Endocam,Pecam1

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES
Anti-CD31 antibody [SPM122] used in IHC (Paraffin sections) (IHC-P). GTX34492

GTX34492 IHC-P Image

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31 Monoclonal Antibody (SPM122)

Package List Price ($)
$ 329