CD31 antibody [SPM532]

Cat. No. GTX34491

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM532
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34491 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant CD31 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD31 , CD31/EndoCAM , GPIIA' , PECA1 , PECAM1 , endoCAM , platelet and endothelial cell adhesion molecule 1

Cellular Localization

Cell membrane,Membrane raft,Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Gene ID: 5175 PECAM1
UniProt: P16284 PECAM1

Research Area

DATA IMAGES

GTX34491 WB Image

WB analysis of Jurkat cell lysate using GTX34491 CD31 antibody [SPM532].

GTX34491 IHC-P Image

IHC-P analysis of human angiosarcoma tissue using GTX34491 CD31 antibody [SPM532].

GTX34491 ICC/IF Image

ICC/IF analysis of PFA-fixed Jurkat cells using GTX34491 CD31 antibody [SPM532].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty