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CD31 antibody [WM59] (PE)

Anti-CD31 antibody [WM59] (PE) used in Flow cytometry (FACS). GTX75302

Cat. No. GTX75302

Reference 22

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 WM59

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Rabbit, Cynomolgus monkey, Rhesus Monkey
Package
100 test ($489)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Product Note

This antibody recognizes the human CD31 antigen. Clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 5% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

RRID

AB_376709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA' , PECA1 , PECAM-1 , endoCAM

Cellular Localization

Cell membrane , Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [WM59] (PE) used in Flow cytometry (FACS). GTX75302

GTX75302 FACS Image

Figure A. FITC conjugated mouse anti human CD11b (GTX76292) and RPE conjugated Mouse IgG1 isotype control (GTX76639). Figure B. FITC conjugated mouse anti human CD11b (GTX76292) and RPE conjugated mouse anti human CD31 (GTX75302). All experiments performed on red cell lysed Human peripheral blood gated on all mononuclear cells

REFERENCE

Clone Reference

Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Yan J et al. J Neuroinflammation 2022; 19 (1):289 TREM2 activation alleviates neural damage via Akt/CREB/BDNF signalling after traumatic brain injury in mice.
Xiaopeng Guo et al. Oxid Med Cell Longev 2022; 2022:7541411 Reducing the Damage of Ox-LDL/LOX-1 Pathway to Vascular Endothelial Barrier Can Inhibit Atherosclerosis.
Xuehui Fan et al. Front Pharmacol 2022; 13:834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Xu H et al. Stem Cells Transl Med 2021; 10 (5):781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
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REVIEW

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Clone Reference
Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Yan J et al. J Neuroinflammation 2022; 19 (1):289 TREM2 activation alleviates neural damage via Akt/CREB/BDNF signalling after traumatic brain injury in mice.
Xiaopeng Guo et al. Oxid Med Cell Longev 2022; 2022:7541411 Reducing the Damage of Ox-LDL/LOX-1 Pathway to Vascular Endothelial Barrier Can Inhibit Atherosclerosis.
Xuehui Fan et al. Front Pharmacol 2022; 13:834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Wang Z et al. Cell Biol Int 2021; (Epub) Exosomal microRNA-1246 from human umbilical cord mesenchymal stem cells potentiates myocardial angiogenesis in chronic heart failure.
Xu H et al. Stem Cells Transl Med 2021; 10 (5):781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
Choo SY et al. Int J Oncol 2019; 54 (4):1327-1336 Runx3 inhibits endothelial progenitor cell differentiation and function via suppression of HIF-1α activity.
Trivedi A et al. J Transl Med 2019; 17 (1):128 Bone marrow donor selection and characterization of MSCs is critical for pre-clinical and clinical cell dose production.
B. Glueck et al. Journal of Immunology Research 2018; 2018:Article ID 9671919 Tributyrin Supplementation Protects Immune Responses and Vasculature and Reduces Oxidative Stress in the Proximal Colon of Mice Exposed to Chronic-Binge Ethanol Feeding.
Ou Y et al. Int J Clin Exp Pathol 2018; 11 (6):3052-3060 Silencing profilin-1 confers protection from oxLDL injury in human vascular endothelial cells.
Chen J et al. Acta Biomater 2018; 80:154-168 Chitosan/silk fibroin modified nanofibrous patches with mesenchymal stem cells prevent heart remodeling post-myocardial infarction in rats.
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Chen PS et al. Sci Rep 2017; 7 (1):3284 Recombinant Thrombomodulin Exerts Anti-autophagic Action in Endothelial Cells and Provides Anti-atherosclerosis Effect in Apolipoprotein E Deficient Mice.
Hsu PY et al. J Cell Mol Med 2016; (Epub) MicroRNA let-7g possesses a therapeutic potential for peripheral artery disease.
Aldahmash A et al. Saudi J Biol Sci 2016; (Epub) Transplantation of human neonatal foreskin stromal cells in ex vivo organotypic cultures of embryonic chick femurs.
Kilarski WW et al. Angiogenesis 2014; 17 (2):347-57 Optimization and regeneration kinetics of lymphatic-specific photodynamic therapy in the mouse dermis.
Chen YJ et al. Gynecol Oncol 2013; 131 (3):734-43 The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Liu YW et al. Int J Cardiol 2013; 168 (1):66-75 Xenografted human amniotic fluid-derived stem cell as a cell source in therapeutic angiogenesis.
Kanczler JM et al. Tissue Eng Part C Methods 2012; 18 (10):747-60 A novel approach for studying the temporal modulation of embryonic skeletal development using organotypic bone cultures and microcomputed tomography.
Shi Q et al. Gene Ther 2010; 17 (9):1085-97 A combinatorial approach for targeted delivery using small molecules and reversible masking to bypass nonspecific uptake in vivo.
Application : FACS
Reactivity : Human
Daussin FN et al. Am J Physiol Regul Integr Comp Physiol 2008; 295 (1):R264-72 Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects.
Breslin JW et al. Lymphat Res Biol 2007; 5 (2):105-13 VEGF-C alters barrier function of cultured lymphatic endothelial cells through a VEGFR-3-dependent mechanism.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489