APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:300-1:5000 |
| 1:50-1:200 |
| 1:200-1:400 |
| 1:50-1:200 |
| 1:20-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
10mM TBS, 1% BSA, 50% Glycerol
Preservative
0.03% Proclin300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide derived from human CD31 (aa 681-738)
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA'' , PECA1 , PECAM-1 , endoCAM
Cellular Localization
Cell membrane , Cell junction
Background
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
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GTX04325 FACS Image
FACS analysis of U937 cells using GTX04325 CD31 antibody.
Green : Primary antibody
Orange : Isotype control
Light blue : Secondary antibody only
Blue : Control
Dilution : 1:20
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GTX04325 WB Image
WB analysis of U937 cell lysates using GTX04325 CD31 antibody.
Dilution : 1:300
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GTX04325 FACS Image
FACS analysis of Jurkat cells using GTX04325 CD31 antibody..
Green : Primary antibody
Orange : Isotype control
Light blue : Secondary antibody only
Blue : Control
Dilution : 1:100
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GTX04325 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04325 CD31 antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200
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GTX04325 WB Image
WB analysis of various tissue lysates using GTX04325 CD31 antibody.
Dilution : 1:1000
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GTX04325 IHC-P Image
IHC-P analysis of human colon cancer tissue using GTX04325 CD31 antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200
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GTX04325 IHC-P Image
IHC-P analysis of human liver tissue using GTX04325 CD31 antibody.
Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15min
Dilution : 1:200
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REFERENCE
There are currently no references for CD31 antibody (GTX04325). Be the first to share your publications with this product.
REVIEW
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